Tracking the transition to agriculture in Southern Europe through ancient DNA analysis of dental calculus
2021
Аутори
Ottoni, ClaudioBorić, Dušan
Cheronet, Olivia
Sparacello, Vitale
Dori, Irene
Coppa, Alfredo
Antonović, Dragana
Vujević, Dario
Price, Douglas T.
Pinhasi, Ron
Cristiani, Emanuela
Чланак у часопису (Објављена верзија)
Метаподаци
Приказ свих података о документуАпстракт
Archaeological dental calculus, or mineralized plaque, is a key tool to track the evolution of oral microbiota across time in response to processes that impacted our culture and biology, such as the rise of farming during the Neolithic. However, the extent to which the human oral flora changed from prehistory until present has remained elusive due to the scarcity of data on the microbiomes of prehistoric humans. Here, we present our reconstruction of oral microbiomes via shotgun metagenomics of dental calculus in 44 ancient foragers and farmers from two regions playing a pivotal role in the spread of farming across Europe-the Balkans and the Italian Peninsula. We show that the introduction of farming in Southern Europe did not alter significantly the oral microbiomes of local forager groups, and it was in particular associated with a higher abundance of the species Olsenella sp. oral taxon 807. The human oral environment in prehistory was dominated by a microbial species, Anaerolineace...ae bacterium oral taxon 439, that diversified geographically. A Near Eastern lineage of this bacterial commensal dispersed with Neolithic farmers and replaced the variant present in the local foragers. Our findings also illustrate that major taxonomic shifts in human oral microbiome composition occurred after the Neolithic and that the functional profile of modern humans evolved in recent times to develop peculiar mechanisms of antibiotic resistance that were previously absent.
Кључне речи:
Southern Europe / metagenomics / dental calculus / ancient DNAИзвор:
Proceedings of the National Academy of Sciences of the United States of America, 2021, 118, 32Издавач:
- Natl Acad Sciences, Washington
Финансирање / пројекти:
- Elixir-Italy
- HPC"CINECA program
- Research Council of NorwayResearch Council of Norway
- Southeastern Regional Health Authorities
- HIDDEN FOODS: Plant foods in Palaeolithic and Mesolithic societies of SE Europe and Italy (EU-639286)
- NSFNational Science Foundation (NSF) [BCS-0235465]
- NOMIS Foundation
- French State of the IdEx Program Bordeaux [ANR-10-IDEX-03-02]
- European Union's Horizon 2020 research and innovation program under the Marie Sklodowska-Curie Grant [752626]
- Croatian Science Foundation [IP-2019-04-6115]
DOI: 10.1073/pnas.2102116118
ISSN: 0027-8424
PubMed: 34312252
WoS: 000685043400014
Scopus: 2-s2.0-85111802200
Институција/група
Археолошки институт / Institute of ArchaeologyTY - JOUR AU - Ottoni, Claudio AU - Borić, Dušan AU - Cheronet, Olivia AU - Sparacello, Vitale AU - Dori, Irene AU - Coppa, Alfredo AU - Antonović, Dragana AU - Vujević, Dario AU - Price, Douglas T. AU - Pinhasi, Ron AU - Cristiani, Emanuela PY - 2021 UR - http://rai.ai.ac.rs/handle/123456789/372 AB - Archaeological dental calculus, or mineralized plaque, is a key tool to track the evolution of oral microbiota across time in response to processes that impacted our culture and biology, such as the rise of farming during the Neolithic. However, the extent to which the human oral flora changed from prehistory until present has remained elusive due to the scarcity of data on the microbiomes of prehistoric humans. Here, we present our reconstruction of oral microbiomes via shotgun metagenomics of dental calculus in 44 ancient foragers and farmers from two regions playing a pivotal role in the spread of farming across Europe-the Balkans and the Italian Peninsula. We show that the introduction of farming in Southern Europe did not alter significantly the oral microbiomes of local forager groups, and it was in particular associated with a higher abundance of the species Olsenella sp. oral taxon 807. The human oral environment in prehistory was dominated by a microbial species, Anaerolineaceae bacterium oral taxon 439, that diversified geographically. A Near Eastern lineage of this bacterial commensal dispersed with Neolithic farmers and replaced the variant present in the local foragers. Our findings also illustrate that major taxonomic shifts in human oral microbiome composition occurred after the Neolithic and that the functional profile of modern humans evolved in recent times to develop peculiar mechanisms of antibiotic resistance that were previously absent. PB - Natl Acad Sciences, Washington T2 - Proceedings of the National Academy of Sciences of the United States of America T1 - Tracking the transition to agriculture in Southern Europe through ancient DNA analysis of dental calculus IS - 32 VL - 118 DO - 10.1073/pnas.2102116118 ER -
@article{ author = "Ottoni, Claudio and Borić, Dušan and Cheronet, Olivia and Sparacello, Vitale and Dori, Irene and Coppa, Alfredo and Antonović, Dragana and Vujević, Dario and Price, Douglas T. and Pinhasi, Ron and Cristiani, Emanuela", year = "2021", abstract = "Archaeological dental calculus, or mineralized plaque, is a key tool to track the evolution of oral microbiota across time in response to processes that impacted our culture and biology, such as the rise of farming during the Neolithic. However, the extent to which the human oral flora changed from prehistory until present has remained elusive due to the scarcity of data on the microbiomes of prehistoric humans. Here, we present our reconstruction of oral microbiomes via shotgun metagenomics of dental calculus in 44 ancient foragers and farmers from two regions playing a pivotal role in the spread of farming across Europe-the Balkans and the Italian Peninsula. We show that the introduction of farming in Southern Europe did not alter significantly the oral microbiomes of local forager groups, and it was in particular associated with a higher abundance of the species Olsenella sp. oral taxon 807. The human oral environment in prehistory was dominated by a microbial species, Anaerolineaceae bacterium oral taxon 439, that diversified geographically. A Near Eastern lineage of this bacterial commensal dispersed with Neolithic farmers and replaced the variant present in the local foragers. Our findings also illustrate that major taxonomic shifts in human oral microbiome composition occurred after the Neolithic and that the functional profile of modern humans evolved in recent times to develop peculiar mechanisms of antibiotic resistance that were previously absent.", publisher = "Natl Acad Sciences, Washington", journal = "Proceedings of the National Academy of Sciences of the United States of America", title = "Tracking the transition to agriculture in Southern Europe through ancient DNA analysis of dental calculus", number = "32", volume = "118", doi = "10.1073/pnas.2102116118" }
Ottoni, C., Borić, D., Cheronet, O., Sparacello, V., Dori, I., Coppa, A., Antonović, D., Vujević, D., Price, D. T., Pinhasi, R.,& Cristiani, E.. (2021). Tracking the transition to agriculture in Southern Europe through ancient DNA analysis of dental calculus. in Proceedings of the National Academy of Sciences of the United States of America Natl Acad Sciences, Washington., 118(32). https://doi.org/10.1073/pnas.2102116118
Ottoni C, Borić D, Cheronet O, Sparacello V, Dori I, Coppa A, Antonović D, Vujević D, Price DT, Pinhasi R, Cristiani E. Tracking the transition to agriculture in Southern Europe through ancient DNA analysis of dental calculus. in Proceedings of the National Academy of Sciences of the United States of America. 2021;118(32). doi:10.1073/pnas.2102116118 .
Ottoni, Claudio, Borić, Dušan, Cheronet, Olivia, Sparacello, Vitale, Dori, Irene, Coppa, Alfredo, Antonović, Dragana, Vujević, Dario, Price, Douglas T., Pinhasi, Ron, Cristiani, Emanuela, "Tracking the transition to agriculture in Southern Europe through ancient DNA analysis of dental calculus" in Proceedings of the National Academy of Sciences of the United States of America, 118, no. 32 (2021), https://doi.org/10.1073/pnas.2102116118 . .